Furhan Iqbal
Bahauddin Zakariya University Pakistan
Title: Creatine monohydrate supplementation for 10 weeks has a potential to improve learning and memory in female albino mice following neonatal hypoxia ischemia encephalopathy
Biography
Biography: Furhan Iqbal
Abstract
Currently there are no uniform standard treatments for newborn suffering from cerebral hypoxia-ischemia (HI) and to find new and effective strategies for treating the HI injury remains a key direction for future research. Present study was designed to demonstrate that optimal dose (1 or 3%) of Creatine monohydrate (Cr) for the treatment of neonatal HI in female albino mice. On postnatal day 10, animals were subjected to left carotid artery ligation followed by 8% hypoxia for 25 minutes. Following weaning on postnatal day 20, mice were divided into three treatments on the basis of diet supplementation (Normal rodent diet, 1% and 3% creatine supplemented diet) for 10 week. A battery of neurological tests (Rota rod, open field and Morris water maze) was used to demonstrate effect of Cr supplementation on neurofunction and infarct size following HI.
Open field test results indicated that Cr supplementation had significantly improved locomotory and exploratory behaviour in subjects. It was observed that Cr treated mice showed better neuromuscular coordination (rota rod) and improved spatial memory (Morris Water Maze test). A significant affect of creatine supplementation in reducing infarct size was also observed. Post hoc analysis of post hoc multiple comparisons revealed that mice supplemented with 3% Cr for 10 weeks performed better during Morris water maze test while 1% Cr supplementation improved the exploratory behaviour and gain in body weight than control group indicating that Cr supplementation has the potential to improve the neurofunction following neonatal brain damage.